{"id":3372,"date":"2023-04-15T01:07:44","date_gmt":"2023-04-14T23:07:44","guid":{"rendered":"https:\/\/web-frankfurt.de\/Sani\/?p=3372"},"modified":"2025-07-25T17:55:08","modified_gmt":"2025-07-25T15:55:08","slug":"intrahippocampal-transplantation-of-dental-pulp-stem-cells-improved-memory-function-and-reduced-neuroinflammation-induced-cell-death-in-the-rats-model-of-seizure","status":"publish","type":"post","link":"https:\/\/web-frankfurt.de\/Sani\/scientific-articles\/intrahippocampal-transplantation-of-dental-pulp-stem-cells-improved-memory-function-and-reduced-neuroinflammation-induced-cell-death-in-the-rats-model-of-seizure\/","title":{"rendered":"Intrahippocampal transplantation of dental pulp stem cells improved memory function and reduced neuroinflammation-induced cell death in the rat\u2019s model of seizure"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"3372\" class=\"elementor elementor-3372\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-206d55ad e-flex e-con-boxed e-con e-parent\" data-id=\"206d55ad\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-4b539a12 elementor-widget elementor-widget-text-editor\" data-id=\"4b539a12\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<blockquote class=\"wp-block-quote\"><p><strong>Abstract and Figures<\/strong><\/p><div><div class=\"nova-legacy-e-text nova-legacy-e-text--size-m nova-legacy-e-text--family-sans-serif nova-legacy-e-text--spacing-xxs nova-legacy-e-text--color-grey-700\"><ul class=\"nova-legacy-e-list nova-legacy-e-list--size-m nova-legacy-e-list--type-inline nova-legacy-e-list--spacing-none\"><li class=\"nova-legacy-e-list__item\">April 2023<\/li><\/ul><\/div><\/div><div>DOI: <a href=\"http:\/\/dx.doi.org\/10.21203\/rs.3.rs-2814327\/v1\" target=\"_blank\" rel=\"noopener\">10.21203\/rs.3.rs-2814327\/v1<\/a><br \/>License <a href=\"https:\/\/www.researchgate.net\/deref\/https%3A%2F%2Fcreativecommons.org%2Flicenses%2Fby%2F4.0%2F?_tp=eyJjb250ZXh0Ijp7ImZpcnN0UGFnZSI6InByb2ZpbGUiLCJwYWdlIjoicHVibGljYXRpb24iLCJwcmV2aW91c1BhZ2UiOiJwcm9maWxlIn19\" target=\"_blank\" rel=\"noopener\">CC BY 4.0<\/a><\/div><h6 style=\"text-align: justify; font-size: 14px; font-weight: 400; line-height: 28px;\">Background Epilepsy is a prevalent neurological disorder that significantly reduces the patient&#8217;s quality of life. The present study aims to evaluate whether dental pulp stem cells (DPSCs) transplant effectively decreases inflammation and cell death in the brain cells to reduce seizure damage. Methods A seizure was induced in rats using intraperitoneal injections of pentylenetetrazole (PTZ). In the PTZ + DPSC group, bilateral hippocampal transplantation of DPSCs in PTZ-lesioned rat models was conducted. After one-month, post-graft analysis was performed, and some behavioral factors, such as working memory and long-term memory, were measured using a T-maze test and passive avoidance test, respectively. We investigated the immunohistopathology and distribution of astrocyte cells through light microscopy and Sholl analysis, respectively. Also, the Voronoi tessellation method was employed to estimate the spatial distribution of the cells in the hippocampus. Results For improving the behavioral aspects of rats with induced seizures, a reduction in astrogliosis, astrocytes process length, the number of branches, and intersections distal to the soma was observed in their hippocampus compared to the control group. More analysis indicated that the grafted DPSCs decreased the caspase-3 expression in the hippocampus of rats with induced seizures. Moreover, the DPSCs transplant protected hippocampal pyramidal neurons against PTZ toxicity and improved the spatial distribution of the hippocampal neurons. Conclusions Our findings suggested that DPSCs transplant can be a potent modifier of astrocytes&#8217; reactivation and inflammatory responses.<\/h6><\/blockquote><blockquote class=\"wp-block-quote\"><p><strong>Authors<\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul><li><a href=\"https:\/\/www.researchgate.net\/profile\/Mojtaba-Sani-2?_tp=eyJjb250ZXh0Ijp7ImZpcnN0UGFnZSI6InByb2ZpbGUiLCJwYWdlIjoicHVibGljYXRpb24iLCJwcmV2aW91c1BhZ2UiOiJwcm9maWxlIn19\">Dr. Dr. Mojtaba Sani<\/a><\/li><li><a class=\"nova-legacy-e-link nova-legacy-e-link--color-inherit nova-legacy-e-link--theme-bare\" href=\"https:\/\/www.researchgate.net\/profile\/Abbas-Aliaghaei?_tp=eyJjb250ZXh0Ijp7ImZpcnN0UGFnZSI6InByb2ZpbGUiLCJwYWdlIjoicHVibGljYXRpb24iLCJwcmV2aW91c1BhZ2UiOiJwcm9maWxlIn19\"><span class=\"publication-header-author__font-weight-normal\">Dr. Abbas Aliaghaei<\/span><\/a><\/li><\/ul><\/li><\/ul><\/blockquote><blockquote class=\"wp-block-quote\"><p><strong>Source Citation<\/strong><\/p><p><a href=\"https:\/\/www.researchgate.net\/publication\/370821708_Intrahippocampal_transplantation_of_dental_pulp_stem_cells_improved_memory_function_and_reduced_neuroinflammation-induced_cell_death_in_the_rat's_model_of_seizure\" target=\"_blank\" rel=\"nofollow noopener\"> <button style=\"padding: 10px 20px; font-size: 16px; background-color: #0c3a2e; color: white; border: none; border-radius: 6px; cursor: pointer;\"> Visit Source <\/button> <\/a><\/p><\/blockquote>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9f2a2d5 elementor-widget elementor-widget-gallery\" data-id=\"9f2a2d5\" data-element_type=\"widget\" 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href=\"https:\/\/web-frankfurt.de\/Sani\/wp-content\/uploads\/2023\/04\/Intrahippocampal-Transplantation-of-Dental-Pulp-Stem-Cells-\u2013-Dr.-Dr.-Sani-Hannover.webp\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"9f2a2d5\" data-elementor-lightbox-title=\"Intrahippocampal-Transplantation-of-Dental-Pulp-Stem-Cells-\u2013-Dr.-Dr.-Sani-Hannover\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6MzM4MiwidXJsIjoiaHR0cHM6XC9cL3dlYi1mcmFua2Z1cnQuZGVcL1NhbmlcL3dwLWNvbnRlbnRcL3VwbG9hZHNcLzIwMjNcLzA0XC9JbnRyYWhpcHBvY2FtcGFsLVRyYW5zcGxhbnRhdGlvbi1vZi1EZW50YWwtUHVscC1TdGVtLUNlbGxzLVx1MjAxMy1Eci4tRHIuLVNhbmktSGFubm92ZXIud2VicCIsInNsaWRlc2hvdyI6IjlmMmEyZDUifQ%3D%3D\">\n\t\t\t\t\t<div class=\"e-gallery-image elementor-gallery-item__image\" data-thumbnail=\"https:\/\/web-frankfurt.de\/Sani\/wp-content\/uploads\/2023\/04\/Intrahippocampal-Transplantation-of-Dental-Pulp-Stem-Cells-\u2013-Dr.-Dr.-Sani-Hannover.webp\" data-width=\"756\" data-height=\"1044\" aria-label=\"Stem cell injection into the hippocampus to treat epilepsy-induced damage \u2013 research by Dr. Sani in Hannover\" role=\"img\" ><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-gallery-item__overlay\"><\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<a class=\"e-gallery-item elementor-gallery-item elementor-animated-content\" href=\"https:\/\/web-frankfurt.de\/Sani\/wp-content\/uploads\/2023\/04\/Memory-Improvement-after-DPSC-Therapy-in-Rat-Seizure-Model-\u2013-Behavioral-Test-Results.webp\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"9f2a2d5\" data-elementor-lightbox-title=\"Memory-Improvement-after-DPSC-Therapy-in-Rat-Seizure-Model-\u2013-Behavioral-Test-Results\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6MzM4MywidXJsIjoiaHR0cHM6XC9cL3dlYi1mcmFua2Z1cnQuZGVcL1NhbmlcL3dwLWNvbnRlbnRcL3VwbG9hZHNcLzIwMjNcLzA0XC9NZW1vcnktSW1wcm92ZW1lbnQtYWZ0ZXItRFBTQy1UaGVyYXB5LWluLVJhdC1TZWl6dXJlLU1vZGVsLVx1MjAxMy1CZWhhdmlvcmFsLVRlc3QtUmVzdWx0cy53ZWJwIiwic2xpZGVzaG93IjoiOWYyYTJkNSJ9\">\n\t\t\t\t\t<div class=\"e-gallery-image elementor-gallery-item__image\" data-thumbnail=\"https:\/\/web-frankfurt.de\/Sani\/wp-content\/uploads\/2023\/04\/Memory-Improvement-after-DPSC-Therapy-in-Rat-Seizure-Model-\u2013-Behavioral-Test-Results.webp\" data-width=\"850\" data-height=\"936\" aria-label=\"Decreased astrogliosis and inflammation in the hippocampus after DPSC therapy \u2013 seizure model study in Hannover\" role=\"img\" ><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-gallery-item__overlay\"><\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<a class=\"e-gallery-item elementor-gallery-item elementor-animated-content\" href=\"https:\/\/web-frankfurt.de\/Sani\/wp-content\/uploads\/2023\/04\/Reduction-of-Astrocyte-Reactivity-and-Neuroinflammation-after-DPSC-Treatment.webp\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"9f2a2d5\" data-elementor-lightbox-title=\"Reduction-of-Astrocyte-Reactivity-and-Neuroinflammation-after-DPSC-Treatment\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6MzM4NCwidXJsIjoiaHR0cHM6XC9cL3dlYi1mcmFua2Z1cnQuZGVcL1NhbmlcL3dwLWNvbnRlbnRcL3VwbG9hZHNcLzIwMjNcLzA0XC9SZWR1Y3Rpb24tb2YtQXN0cm9jeXRlLVJlYWN0aXZpdHktYW5kLU5ldXJvaW5mbGFtbWF0aW9uLWFmdGVyLURQU0MtVHJlYXRtZW50LndlYnAiLCJzbGlkZXNob3ciOiI5ZjJhMmQ1In0%3D\">\n\t\t\t\t\t<div class=\"e-gallery-image elementor-gallery-item__image\" data-thumbnail=\"https:\/\/web-frankfurt.de\/Sani\/wp-content\/uploads\/2023\/04\/Reduction-of-Astrocyte-Reactivity-and-Neuroinflammation-after-DPSC-Treatment.webp\" data-width=\"803\" data-height=\"550\" aria-label=\"Improved working and long-term memory in rats after dental pulp stem cell transplant \u2013 Dr. Sani&#039;s neuroscience research in Hannover\" role=\"img\" ><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-gallery-item__overlay\"><\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c871d3f elementor-widget elementor-widget-text-editor\" data-id=\"c871d3f\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"nova-legacy-e-text nova-legacy-e-text--size-l nova-legacy-e-text--family-sans-serif nova-legacy-e-text--spacing-xxs nova-legacy-e-text--color-grey-700 research-detail-figures-legal\" style=\"text-align: center;\">Figures &#8211; available via license:\u00a0<a class=\"nova-legacy-e-link nova-legacy-e-link--color-inherit nova-legacy-e-link--theme-decorated\" href=\"https:\/\/creativecommons.org\/licenses\/by\/4.0\/\">Creative Commons Attribution 4.0 International<\/a><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Abstract and Figures April 2023 DOI: 10.21203\/rs.3.rs-2814327\/v1License CC BY 4.0 Background Epilepsy is a prevalent neurological disorder that significantly reduces the patient&#8217;s quality of life. The present study aims to evaluate whether dental pulp stem cells (DPSCs) transplant effectively decreases inflammation and cell death in the brain cells to reduce seizure damage. Methods A seizure <\/p>\n","protected":false},"author":1,"featured_media":2815,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[61,46],"tags":[],"class_list":["post-3372","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-neuroscience-regenerative-medicine","category-scientific-articles"],"_links":{"self":[{"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/posts\/3372","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/comments?post=3372"}],"version-history":[{"count":28,"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/posts\/3372\/revisions"}],"predecessor-version":[{"id":3682,"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/posts\/3372\/revisions\/3682"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/media\/2815"}],"wp:attachment":[{"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/media?parent=3372"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/categories?post=3372"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/tags?post=3372"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}