{"id":3571,"date":"2023-10-25T16:49:38","date_gmt":"2023-10-25T14:49:38","guid":{"rendered":"https:\/\/web-frankfurt.de\/Sani\/?p=3571"},"modified":"2025-07-25T18:10:55","modified_gmt":"2025-07-25T16:10:55","slug":"grafted-sertoli-cells-prevent-neuronal-cell-death-and-memory-loss-induced-by-seizures","status":"publish","type":"post","link":"https:\/\/web-frankfurt.de\/Sani\/scientific-articles\/grafted-sertoli-cells-prevent-neuronal-cell-death-and-memory-loss-induced-by-seizures\/","title":{"rendered":"Grafted Sertoli cells prevent neuronal cell death and memory loss induced by seizures"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"3571\" class=\"elementor elementor-3571\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-206d55ad e-flex e-con-boxed e-con e-parent\" data-id=\"206d55ad\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-4b539a12 elementor-widget elementor-widget-text-editor\" data-id=\"4b539a12\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<blockquote class=\"wp-block-quote\"><p><strong>Abstract and Figures<\/strong><\/p><div class=\"nova-legacy-e-text nova-legacy-e-text--size-m nova-legacy-e-text--family-sans-serif nova-legacy-e-text--spacing-xxs nova-legacy-e-text--color-grey-700\"><ul class=\"nova-legacy-e-list nova-legacy-e-list--size-m nova-legacy-e-list--type-inline nova-legacy-e-list--spacing-none\"><li class=\"nova-legacy-e-list__item\"><div class=\"nova-legacy-e-text nova-legacy-e-text--size-m nova-legacy-e-text--family-sans-serif nova-legacy-e-text--spacing-xxs nova-legacy-e-text--color-grey-700\"><ul class=\"nova-legacy-e-list nova-legacy-e-list--size-m nova-legacy-e-list--type-inline nova-legacy-e-list--spacing-none\"><li class=\"nova-legacy-e-list__item\"><div class=\"research-detail-header-section__metadata-after-square-logo\"><div class=\"nova-legacy-e-text nova-legacy-e-text--size-m nova-legacy-e-text--family-sans-serif nova-legacy-e-text--spacing-xxs nova-legacy-e-text--color-grey-700\"><ul class=\"nova-legacy-e-list nova-legacy-e-list--size-m nova-legacy-e-list--type-inline nova-legacy-e-list--spacing-none\"><li class=\"nova-legacy-e-list__item\">October 2023<\/li><li class=\"nova-legacy-e-list__item\"><a class=\"nova-legacy-e-link nova-legacy-e-link--color-inherit nova-legacy-e-link--theme-decorated\" href=\"https:\/\/www.researchgate.net\/journal\/Metabolic-Brain-Disease-1573-7365\">Metabolic Brain Disease<\/a>\u00a038(8):1-16<\/li><\/ul><\/div><div class=\"nova-legacy-e-text nova-legacy-e-text--size-m nova-legacy-e-text--family-sans-serif nova-legacy-e-text--spacing-xxs nova-legacy-e-text--color-grey-700\">DOI: <a class=\"nova-legacy-e-link nova-legacy-e-link--color-inherit nova-legacy-e-link--theme-decorated\" href=\"http:\/\/dx.doi.org\/10.1007\/s11011-023-01309-0\" target=\"_blank\" rel=\"noopener\">10.1007\/s11011-023-01309-0<\/a><br \/><br \/><\/div><\/div>Epilepsy significantly reduces the patient\u2019s quality of life, and we still need to develop new therapeutic approaches to control it. Transplantation of cells such as Sertoli cells (SCs), having a potent ability to release a variety of growth and immunoprotective substances, have made them a potential candidate to deal with neurological diseases like epilepsy. Hence, this study aims to evaluate whether SCs transplant effectively protects the hippocampus astrocytes and neurons to oppose seizure damage. For this purpose, the effects of bilateral intrahippocampal transplantation of SCs were investigated on the rats with the pentylenetetrazol (PTZ) induced seizure. After one-month, post-graft analysis was performed regarding behavior, immunohistopathology, and the distribution of the hippocampal cells. Our findings showed SCs transplantation reduced astrogliosis, astrocytes process length, the number of branches, and intersections distal to the soma of the hippocampus in the seizure group. In rats with grafted SCs, there was a drop in the hippocampal caspase-3 expression. Moreover, the SCs showed another protective impact, as shown by an improvement in pyramidal neurons\u2019 number and spatial distribution. The findings suggested that SCs transplantation can potently modify astrocytes\u2019 reactivation and inflammatory responses.<\/li><\/ul><\/div><\/li><\/ul><\/div><\/blockquote><blockquote class=\"wp-block-quote\"><p><strong>Authors<\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul><li><a href=\"https:\/\/www.researchgate.net\/profile\/Mojtaba-Sani-2?_tp=eyJjb250ZXh0Ijp7ImZpcnN0UGFnZSI6InByb2ZpbGUiLCJwYWdlIjoicHVibGljYXRpb24iLCJwcmV2aW91c1BhZ2UiOiJwcm9maWxlIn19\">Dr. Dr. Mojtaba Sani<\/a><\/li><li><a class=\"nova-legacy-e-link nova-legacy-e-link--color-inherit nova-legacy-e-link--theme-bare\" href=\"https:\/\/www.researchgate.net\/profile\/Abbas-Aliaghaei?_tp=eyJjb250ZXh0Ijp7ImZpcnN0UGFnZSI6InByb2ZpbGUiLCJwYWdlIjoicHVibGljYXRpb24iLCJwcmV2aW91c1BhZ2UiOiJwcm9maWxlIn19\"><span class=\"publication-header-author__font-weight-normal\">Dr. Abbas Aliaghaei<\/span><\/a><\/li><\/ul><\/li><\/ul><\/blockquote><blockquote class=\"wp-block-quote\"><p><strong>Source Citation<\/strong><\/p><p><a href=\"https:\/\/www.researchgate.net\/publication\/374810922_Grafted_Sertoli_cells_prevent_neuronal_cell_death_and_memory_loss_induced_by_seizures\" target=\"_blank\" rel=\"nofollow noopener\"> <button style=\"padding: 10px 20px; font-size: 16px; background-color: #0c3a2e; color: white; border: none; border-radius: 6px; cursor: pointer;\"> Visit Source <\/button> <\/a><\/p><\/blockquote>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9f2a2d5 elementor-widget elementor-widget-gallery\" data-id=\"9f2a2d5\" data-element_type=\"widget\" data-settings=\"{&quot;columns&quot;:3,&quot;gap&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:15,&quot;sizes&quot;:[]},&quot;lazyload&quot;:&quot;yes&quot;,&quot;gallery_layout&quot;:&quot;grid&quot;,&quot;columns_tablet&quot;:2,&quot;columns_mobile&quot;:1,&quot;gap_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:10,&quot;sizes&quot;:[]},&quot;gap_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:10,&quot;sizes&quot;:[]},&quot;link_to&quot;:&quot;file&quot;,&quot;aspect_ratio&quot;:&quot;3:2&quot;,&quot;overlay_background&quot;:&quot;yes&quot;,&quot;content_hover_animation&quot;:&quot;fade-in&quot;}\" data-widget_type=\"gallery.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-gallery__container\">\n\t\t\t\t\t\t\t<a class=\"e-gallery-item elementor-gallery-item elementor-animated-content\" href=\"https:\/\/web-frankfurt.de\/Sani\/wp-content\/uploads\/2025\/07\/Effect-of-SCs-transplant-on-the-expression-of-the-caspase-3-in-hippocampus-and-analyze.webp\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"9f2a2d5\" data-elementor-lightbox-title=\"Effect-of-SCs-transplant-on-the-expression-of-the-caspase-3-in-hippocampus-and-analyze\" data-elementor-lightbox-description=\"Effect of SCs transplant on the expression of the caspase-3 in hippocampus, and analyze of caspase-3 expression in different groups. An increase in caspase-3 expression relative to the control group is clearly detectable in the PTZ one. Caspase-3 expression reduced significantly in PTZ\u2009+\u2009Sertoli cells rats compared to PTZ ones. (**P\u2009&lt;\u20090.01; ***P\u2009&lt;\u20090.001). The values were expressed as means\u2009\u00b1\u2009SD\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6MzU3NCwidXJsIjoiaHR0cHM6XC9cL3dlYi1mcmFua2Z1cnQuZGVcL1NhbmlcL3dwLWNvbnRlbnRcL3VwbG9hZHNcLzIwMjVcLzA3XC9FZmZlY3Qtb2YtU0NzLXRyYW5zcGxhbnQtb24tdGhlLWV4cHJlc3Npb24tb2YtdGhlLWNhc3Bhc2UtMy1pbi1oaXBwb2NhbXB1cy1hbmQtYW5hbHl6ZS53ZWJwIiwic2xpZGVzaG93IjoiOWYyYTJkNSJ9\">\n\t\t\t\t\t<div class=\"e-gallery-image elementor-gallery-item__image\" data-thumbnail=\"https:\/\/web-frankfurt.de\/Sani\/wp-content\/uploads\/2025\/07\/Effect-of-SCs-transplant-on-the-expression-of-the-caspase-3-in-hippocampus-and-analyze.webp\" data-width=\"850\" data-height=\"952\" aria-label=\"Effect of SCs transplant on the expression of the caspase-3 in hippocampus, and analyze of caspase-3 expression in different groups. An increase in caspase-3 expression relative to the control group is clearly detectable in the PTZ one. Caspase-3 expression reduced significantly in PTZ\u2009+\u2009Sertoli cells rats compared to PTZ ones. (**P\u2009\" role=\"img\" ><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-gallery-item__overlay\"><\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<a class=\"e-gallery-item elementor-gallery-item elementor-animated-content\" href=\"https:\/\/web-frankfurt.de\/Sani\/wp-content\/uploads\/2025\/07\/Effect-of-seizure-and-SCs-grafted-on-the-astrogliosis-in-hippocampus-of-rats.webp\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"9f2a2d5\" data-elementor-lightbox-title=\"Effect-of-seizure-and-SCs-grafted-on-the-astrogliosis-in-hippocampus-of-rats\" data-elementor-lightbox-description=\"Effect of seizure and SCs grafted on the astrogliosis in hippocampus of rats. a Immunohistochemical staining for astrocyte marker (GFAP). As indicated in graph (b), SCs implantation reduced the number of GFAP positive cells in PTZ\u2009+\u2009Sertoli cells compared to the PTZ group. Two quantitative indicators of spatial distribution of astrocytes, NND and regularity index were showed in (c) and (d), respectively. (*P\u2009&lt;\u20090.05; **P\u2009&lt;\u20090.01; ***P\u2009&lt;\u20090.001). The values were expressed as means\u2009\u00b1\u2009SD\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6MzU3NSwidXJsIjoiaHR0cHM6XC9cL3dlYi1mcmFua2Z1cnQuZGVcL1NhbmlcL3dwLWNvbnRlbnRcL3VwbG9hZHNcLzIwMjVcLzA3XC9FZmZlY3Qtb2Ytc2VpenVyZS1hbmQtU0NzLWdyYWZ0ZWQtb24tdGhlLWFzdHJvZ2xpb3Npcy1pbi1oaXBwb2NhbXB1cy1vZi1yYXRzLndlYnAiLCJzbGlkZXNob3ciOiI5ZjJhMmQ1In0%3D\">\n\t\t\t\t\t<div class=\"e-gallery-image elementor-gallery-item__image\" data-thumbnail=\"https:\/\/web-frankfurt.de\/Sani\/wp-content\/uploads\/2025\/07\/Effect-of-seizure-and-SCs-grafted-on-the-astrogliosis-in-hippocampus-of-rats.webp\" data-width=\"850\" data-height=\"626\" aria-label=\"Effect of seizure and SCs grafted on the astrogliosis in hippocampus of rats. a Immunohistochemical staining for astrocyte marker (GFAP). As indicated in graph (b), SCs implantation reduced the number of GFAP positive cells in PTZ\u2009+\u2009Sertoli cells compared to the PTZ group. Two quantitative indicators of spatial distribution of astrocytes, NND and regularity index were showed in (c) and (d), respectively. (*P\u2009\" role=\"img\" ><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-gallery-item__overlay\"><\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<a class=\"e-gallery-item elementor-gallery-item elementor-animated-content\" href=\"https:\/\/web-frankfurt.de\/Sani\/wp-content\/uploads\/2025\/07\/In-vitro-characterization-of-SCs-aA-spindle-shaped-morphology-of-SCs-after-1-week-of.webp\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"9f2a2d5\" data-elementor-lightbox-title=\"In-vitro-characterization-of-SCs-aA-spindle-shaped-morphology-of-SCs-after-1-week-of\" data-elementor-lightbox-description=\"In vitro characterization of SCs. a A spindle-shaped morphology of SCs after 1 week of isolation. b Immunocytochemical staining revealed these cells were immunopositive for GDNF and c FasL. d Hippocampal sections were immunostained with anti FasL. The FasL positive SCs transplant could clearly be seen in the hippocampus\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6MzU3NiwidXJsIjoiaHR0cHM6XC9cL3dlYi1mcmFua2Z1cnQuZGVcL1NhbmlcL3dwLWNvbnRlbnRcL3VwbG9hZHNcLzIwMjVcLzA3XC9Jbi12aXRyby1jaGFyYWN0ZXJpemF0aW9uLW9mLVNDcy1hQS1zcGluZGxlLXNoYXBlZC1tb3JwaG9sb2d5LW9mLVNDcy1hZnRlci0xLXdlZWstb2Yud2VicCIsInNsaWRlc2hvdyI6IjlmMmEyZDUifQ%3D%3D\">\n\t\t\t\t\t<div class=\"e-gallery-image elementor-gallery-item__image\" data-thumbnail=\"https:\/\/web-frankfurt.de\/Sani\/wp-content\/uploads\/2025\/07\/In-vitro-characterization-of-SCs-aA-spindle-shaped-morphology-of-SCs-after-1-week-of.webp\" data-width=\"850\" data-height=\"914\" aria-label=\"In vitro characterization of SCs. a A spindle-shaped morphology of SCs after 1 week of isolation. b Immunocytochemical staining revealed these cells were immunopositive for GDNF and c FasL. d Hippocampal sections were immunostained with anti FasL. The FasL positive SCs transplant could clearly be seen in the hippocampus\" role=\"img\" ><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-gallery-item__overlay\"><\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<a class=\"e-gallery-item elementor-gallery-item elementor-animated-content\" href=\"https:\/\/web-frankfurt.de\/Sani\/wp-content\/uploads\/2025\/07\/Sholl-analysis-findings-aThe-injection-of-PTZ-reduced-the-complexity-of-astrocyte.webp\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"9f2a2d5\" data-elementor-lightbox-title=\"Sholl-analysis-findings-aThe-injection-of-PTZ-reduced-the-complexity-of-astrocyte\" data-elementor-lightbox-description=\"Sholl analysis findings. a The injection of PTZ reduced the complexity of astrocyte processes while the SCs transplant prevented it. b SCs graft increased the total astrocyte process length compared to the PTZ group. c Astrocyte soma size reduced in PTZ\u2009+\u2009Sertoli cells group compared to the PTZ one. d Transplant of SCs increased astrocyte arbors area. e Total number of branches was increased in PTZ\u2009+\u2009Sertoli cells compared to the PTZ group. f Percentage of primary, secondary and tertiary arbors of astrocyte in subjects. (*P\u2009&lt;\u20090.05; **P\u2009&lt;\u20090.01; ***P\u2009&lt;\u20090.001). The values were expressed as means\u2009\u00b1\u2009SD\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6MzU3NywidXJsIjoiaHR0cHM6XC9cL3dlYi1mcmFua2Z1cnQuZGVcL1NhbmlcL3dwLWNvbnRlbnRcL3VwbG9hZHNcLzIwMjVcLzA3XC9TaG9sbC1hbmFseXNpcy1maW5kaW5ncy1hVGhlLWluamVjdGlvbi1vZi1QVFotcmVkdWNlZC10aGUtY29tcGxleGl0eS1vZi1hc3Ryb2N5dGUud2VicCIsInNsaWRlc2hvdyI6IjlmMmEyZDUifQ%3D%3D\">\n\t\t\t\t\t<div class=\"e-gallery-image elementor-gallery-item__image\" data-thumbnail=\"https:\/\/web-frankfurt.de\/Sani\/wp-content\/uploads\/2025\/07\/Sholl-analysis-findings-aThe-injection-of-PTZ-reduced-the-complexity-of-astrocyte.webp\" data-width=\"850\" data-height=\"938\" aria-label=\"Sholl analysis findings. a The injection of PTZ reduced the complexity of astrocyte processes while the SCs transplant prevented it. b SCs graft increased the total astrocyte process length compared to the PTZ group. c Astrocyte soma size reduced in PTZ\u2009+\u2009Sertoli cells group compared to the PTZ one. d Transplant of SCs increased astrocyte arbors area. e Total number of branches was increased in PTZ\u2009+\u2009Sertoli cells compared to the PTZ group. f Percentage of primary, secondary and tertiary arbors of astrocyte in subjects. (*P\u2009\" role=\"img\" ><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-gallery-item__overlay\"><\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<a class=\"e-gallery-item elementor-gallery-item elementor-animated-content\" href=\"https:\/\/web-frankfurt.de\/Sani\/wp-content\/uploads\/2025\/07\/The-effect-of-SCs-implantation-on-short-term-memory-and-long-term-memory-at-30th-day.webp\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"9f2a2d5\" data-elementor-lightbox-title=\"The-effect-of-SCs-implantation-on-short-term-memory-and-long-term-memory-at-30th-day\" data-elementor-lightbox-description=\"The effect of SCs implantation on short term memory and long-term memory at 30th day. a Sample movement traces of a rat in T-maze. b Two important variables of T-maze, the alternation rate (%) (c) and latency as indicators of short-term memory. e Time spent in dark compartment and f step through latency. (*P\u2009&lt;\u20090.05; ***P\u2009&lt;\u20090.001). The values were expressed as means\u2009\u00b1\u2009SD\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6MzU3OCwidXJsIjoiaHR0cHM6XC9cL3dlYi1mcmFua2Z1cnQuZGVcL1NhbmlcL3dwLWNvbnRlbnRcL3VwbG9hZHNcLzIwMjVcLzA3XC9UaGUtZWZmZWN0LW9mLVNDcy1pbXBsYW50YXRpb24tb24tc2hvcnQtdGVybS1tZW1vcnktYW5kLWxvbmctdGVybS1tZW1vcnktYXQtMzB0aC1kYXkud2VicCIsInNsaWRlc2hvdyI6IjlmMmEyZDUifQ%3D%3D\">\n\t\t\t\t\t<div class=\"e-gallery-image elementor-gallery-item__image\" data-thumbnail=\"https:\/\/web-frankfurt.de\/Sani\/wp-content\/uploads\/2025\/07\/The-effect-of-SCs-implantation-on-short-term-memory-and-long-term-memory-at-30th-day.webp\" data-width=\"850\" data-height=\"1144\" aria-label=\"The effect of SCs implantation on short term memory and long-term memory at 30th day. a Sample movement traces of a rat in T-maze. b Two important variables of T-maze, the alternation rate (%) (c) and latency as indicators of short-term memory. e Time spent in dark compartment and f step through latency. (*P\u2009\" role=\"img\" ><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-gallery-item__overlay\"><\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<a class=\"e-gallery-item elementor-gallery-item elementor-animated-content\" href=\"https:\/\/web-frankfurt.de\/Sani\/wp-content\/uploads\/2025\/07\/The-estimation-of-the-number-of-hippocampal-neurons-bThe-effects-of-the-SCs-transplant.webp\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"9f2a2d5\" data-elementor-lightbox-title=\"The-estimation-of-the-number-of-hippocampal-neurons-bThe-effects-of-the-SCs-transplant\" data-elementor-lightbox-description=\"The estimation of the number of hippocampal neurons. b The effects of the SCs transplant on the number of the neurons c and dark neurons. As shown in the graphs, although SCs transplant significantly improved the number of the neurons and it was failed to increase the number of dark neurons. Black arrows marked healthy neurons and black triangle marked dark neurons (*P\u2009&lt;\u20090.05; **P\u2009&lt;\u20090.01; ***P\u2009&lt;\u20090.001). The values were expressed as means\u2009\u00b1\u2009SD\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6MzU3OSwidXJsIjoiaHR0cHM6XC9cL3dlYi1mcmFua2Z1cnQuZGVcL1NhbmlcL3dwLWNvbnRlbnRcL3VwbG9hZHNcLzIwMjVcLzA3XC9UaGUtZXN0aW1hdGlvbi1vZi10aGUtbnVtYmVyLW9mLWhpcHBvY2FtcGFsLW5ldXJvbnMtYlRoZS1lZmZlY3RzLW9mLXRoZS1TQ3MtdHJhbnNwbGFudC53ZWJwIiwic2xpZGVzaG93IjoiOWYyYTJkNSJ9\">\n\t\t\t\t\t<div class=\"e-gallery-image elementor-gallery-item__image\" data-thumbnail=\"https:\/\/web-frankfurt.de\/Sani\/wp-content\/uploads\/2025\/07\/The-estimation-of-the-number-of-hippocampal-neurons-bThe-effects-of-the-SCs-transplant.webp\" data-width=\"850\" data-height=\"726\" aria-label=\"The estimation of the number of hippocampal neurons. b The effects of the SCs transplant on the number of the neurons c and dark neurons. As shown in the graphs, although SCs transplant significantly improved the number of the neurons and it was failed to increase the number of dark neurons. Black arrows marked healthy neurons and black triangle marked dark neurons (*P\u2009\" role=\"img\" ><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-gallery-item__overlay\"><\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<a class=\"e-gallery-item elementor-gallery-item elementor-animated-content\" href=\"https:\/\/web-frankfurt.de\/Sani\/wp-content\/uploads\/2025\/07\/Voronoi-analysis-aA-micrograph-of-cells-and-schematic-of-Voronoi-tessellation-in-the.webp\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"9f2a2d5\" data-elementor-lightbox-title=\"Voronoi-analysis-aA-micrograph-of-cells-and-schematic-of-Voronoi-tessellation-in-the\" data-elementor-lightbox-description=\"Voronoi analysis. a A micrograph of cells and schematic of Voronoi tessellation in the hippocampus of study groups. b Mean\u2009\u00b1\u2009Standard deviation of Voronoi polygon area (%) and c Coefficient of Variation (CV) within the pyramidal layer of hippocampus. (*P\u2009&lt;\u20090.05; **P\u2009&lt;\u20090.01; ***P\u2009&lt;\u20090.001). The values were expressed as means\u2009\u00b1\u2009SD\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6MzU4MCwidXJsIjoiaHR0cHM6XC9cL3dlYi1mcmFua2Z1cnQuZGVcL1NhbmlcL3dwLWNvbnRlbnRcL3VwbG9hZHNcLzIwMjVcLzA3XC9Wb3Jvbm9pLWFuYWx5c2lzLWFBLW1pY3JvZ3JhcGgtb2YtY2VsbHMtYW5kLXNjaGVtYXRpYy1vZi1Wb3Jvbm9pLXRlc3NlbGxhdGlvbi1pbi10aGUud2VicCIsInNsaWRlc2hvdyI6IjlmMmEyZDUifQ%3D%3D\">\n\t\t\t\t\t<div class=\"e-gallery-image elementor-gallery-item__image\" data-thumbnail=\"https:\/\/web-frankfurt.de\/Sani\/wp-content\/uploads\/2025\/07\/Voronoi-analysis-aA-micrograph-of-cells-and-schematic-of-Voronoi-tessellation-in-the.webp\" data-width=\"850\" data-height=\"1096\" aria-label=\"Voronoi analysis. a A micrograph of cells and schematic of Voronoi tessellation in the hippocampus of study groups. b Mean\u2009\u00b1\u2009Standard deviation of Voronoi polygon area (%) and c Coefficient of Variation (CV) within the pyramidal layer of hippocampus. (*P\u2009\" role=\"img\" ><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-gallery-item__overlay\"><\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c871d3f elementor-widget elementor-widget-text-editor\" data-id=\"c871d3f\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"nova-legacy-e-text nova-legacy-e-text--size-l nova-legacy-e-text--family-sans-serif nova-legacy-e-text--spacing-xxs nova-legacy-e-text--color-grey-700 research-detail-figures-legal\" style=\"text-align: center;\">Figures &#8211; available from: Metabolic Brain Disease<\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Abstract and Figures October 2023 Metabolic Brain Disease\u00a038(8):1-16 DOI: 10.1007\/s11011-023-01309-0 Epilepsy significantly reduces the patient\u2019s quality of life, and we still need to develop new therapeutic approaches to control it. Transplantation of cells such as Sertoli cells (SCs), having a potent ability to release a variety of growth and immunoprotective substances, have made them a <\/p>\n","protected":false},"author":1,"featured_media":2815,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[46],"tags":[],"class_list":["post-3571","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-scientific-articles"],"_links":{"self":[{"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/posts\/3571","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/comments?post=3571"}],"version-history":[{"count":7,"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/posts\/3571\/revisions"}],"predecessor-version":[{"id":3699,"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/posts\/3571\/revisions\/3699"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/media\/2815"}],"wp:attachment":[{"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/media?parent=3571"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/categories?post=3571"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/tags?post=3571"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}