{"id":3589,"date":"2023-10-28T16:58:56","date_gmt":"2023-10-28T14:58:56","guid":{"rendered":"https:\/\/web-frankfurt.de\/Sani\/?p=3589"},"modified":"2025-07-25T18:11:39","modified_gmt":"2025-07-25T16:11:39","slug":"exposure-to-%ce%b49-tetrahydrocannabinol-leads-to-a-rise-in-caspase-3-morphological-changes-in-microglial-and-astrocyte-reactivity-in-the-cerebellum-of-rats","status":"publish","type":"post","link":"https:\/\/web-frankfurt.de\/Sani\/scientific-articles\/exposure-to-%ce%b49-tetrahydrocannabinol-leads-to-a-rise-in-caspase-3-morphological-changes-in-microglial-and-astrocyte-reactivity-in-the-cerebellum-of-rats\/","title":{"rendered":"Exposure to \u03949-tetrahydrocannabinol leads to a rise in caspase-3, morphological changes in microglial, and astrocyte reactivity in the cerebellum of rats"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"3589\" class=\"elementor elementor-3589\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-206d55ad e-flex e-con-boxed e-con e-parent\" data-id=\"206d55ad\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-4b539a12 elementor-widget elementor-widget-text-editor\" data-id=\"4b539a12\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<blockquote class=\"wp-block-quote\"><p><strong>Abstract and Figures<\/strong><\/p><div class=\"nova-legacy-e-text nova-legacy-e-text--size-m nova-legacy-e-text--family-sans-serif nova-legacy-e-text--spacing-xxs nova-legacy-e-text--color-grey-700\"><ul class=\"nova-legacy-e-list nova-legacy-e-list--size-m nova-legacy-e-list--type-inline nova-legacy-e-list--spacing-none\"><li class=\"nova-legacy-e-list__item\"><div class=\"nova-legacy-e-text nova-legacy-e-text--size-m nova-legacy-e-text--family-sans-serif nova-legacy-e-text--spacing-xxs nova-legacy-e-text--color-grey-700\"><ul class=\"nova-legacy-e-list nova-legacy-e-list--size-m nova-legacy-e-list--type-inline nova-legacy-e-list--spacing-none\"><li class=\"nova-legacy-e-list__item\"><div class=\"nova-legacy-e-text nova-legacy-e-text--size-m nova-legacy-e-text--family-sans-serif nova-legacy-e-text--spacing-xxs nova-legacy-e-text--color-grey-700\"><div class=\"research-detail-header-section__metadata-after-square-logo\"><div class=\"nova-legacy-e-text nova-legacy-e-text--size-m nova-legacy-e-text--family-sans-serif nova-legacy-e-text--spacing-xxs nova-legacy-e-text--color-grey-700\"><ul class=\"nova-legacy-e-list nova-legacy-e-list--size-m nova-legacy-e-list--type-inline nova-legacy-e-list--spacing-none\"><li class=\"nova-legacy-e-list__item\">October 2023<\/li><li class=\"nova-legacy-e-list__item\"><a class=\"nova-legacy-e-link nova-legacy-e-link--color-inherit nova-legacy-e-link--theme-decorated\" href=\"https:\/\/www.researchgate.net\/journal\/Toxicology-Research-2045-4538\">Toxicology Research<\/a>\u00a012(3)<\/li><\/ul><\/div><div class=\"nova-legacy-e-text nova-legacy-e-text--size-m nova-legacy-e-text--family-sans-serif nova-legacy-e-text--spacing-xxs nova-legacy-e-text--color-grey-700\">DOI: <a class=\"nova-legacy-e-link nova-legacy-e-link--color-inherit nova-legacy-e-link--theme-decorated\" href=\"http:\/\/dx.doi.org\/10.1093\/toxres\/tfad098\" target=\"_blank\" rel=\"noopener\">10.1093\/toxres\/tfad098<\/a><br \/><br \/><\/div><\/div><\/div><div class=\"nova-legacy-l-flex__item nova-legacy-l-flex__item--grow research-detail-middle-section__item\"><div class=\"nova-legacy-c-card nova-legacy-c-card--spacing-m nova-legacy-c-card--elevation-1-above\"><div class=\"nova-legacy-c-card__body nova-legacy-c-card__body--spacing-inherit\"><div class=\"nova-legacy-e-text nova-legacy-e-text--size-m nova-legacy-e-text--family-sans-serif nova-legacy-e-text--spacing-none nova-legacy-e-text--color-grey-800 research-detail-middle-section__abstract\"><div class=\"nova-legacy-l-flex__item nova-legacy-l-flex__item--grow research-detail-middle-section__item\"><div class=\"nova-legacy-c-card nova-legacy-c-card--spacing-m nova-legacy-c-card--elevation-1-above\"><div class=\"nova-legacy-c-card__body nova-legacy-c-card__body--spacing-inherit\"><div class=\"nova-legacy-e-text nova-legacy-e-text--size-m nova-legacy-e-text--family-sans-serif nova-legacy-e-text--spacing-none nova-legacy-e-text--color-grey-800 research-detail-middle-section__abstract\">The present study aimed to elucidate the effect of 10 mg\/kg \u03949-tetrahydrocannabinol (THC) on cerebellar neuronal and glial morphology, apoptosis and inflammatory gene expression using a series of histological assays including stereology, Sholl analysis, immunofluorescence and real-time qPCR in male Wistar rats. A decrease in the number of Purkinje neurons and the thickness of the granular layer in the cerebellum was reported in THC-treated rats. Increased expression of Iba-1 and arborization of microglial processes were evidence of microgliosis and morphological changes in microglia. In addition, astrogliosis and changes in astrocyte morphology were other findings associated with THC administration. THC also led to an increase in caspase-3 positive cells and a decrease in autophagy and inflammatory gene expression such as mTOR, BECN1 and LAMP2. However, there were no significant changes in the volume of molecular layers and white matter, the spatial arrangement of granular layers and white matter, or the spatial arrangement of granular layers and white matter in the cerebellum. Taken together, our data showed both neuroprotective and neurodegenerative properties of THC in the cerebellum, which require further study in the future.<\/div><\/div><\/div><\/div><div class=\"nova-legacy-l-flex__item nova-legacy-l-flex__item--grow research-detail-middle-section__item\">\u00a0<\/div><\/div><\/div><\/div><\/div><\/li><\/ul><\/div><\/li><\/ul><\/div><\/blockquote><blockquote class=\"wp-block-quote\"><p><strong>Authors<\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul><li><a href=\"https:\/\/www.researchgate.net\/profile\/Mojtaba-Sani-2?_tp=eyJjb250ZXh0Ijp7ImZpcnN0UGFnZSI6InByb2ZpbGUiLCJwYWdlIjoicHVibGljYXRpb24iLCJwcmV2aW91c1BhZ2UiOiJwcm9maWxlIn19\">Dr. Dr. Mojtaba Sani<\/a><\/li><li><a class=\"nova-legacy-e-link nova-legacy-e-link--color-inherit nova-legacy-e-link--theme-bare\" href=\"https:\/\/www.researchgate.net\/profile\/Abbas-Aliaghaei?_tp=eyJjb250ZXh0Ijp7ImZpcnN0UGFnZSI6InByb2ZpbGUiLCJwYWdlIjoicHVibGljYXRpb24iLCJwcmV2aW91c1BhZ2UiOiJwcm9maWxlIn19\"><span class=\"publication-header-author__font-weight-normal\">Dr. Abbas Aliaghaei<\/span><\/a><\/li><\/ul><\/li><\/ul><\/blockquote><blockquote class=\"wp-block-quote\"><p><strong>Source Citation<\/strong><\/p><p><a href=\"https:\/\/www.researchgate.net\/publication\/375121734_Exposure_to_D9-tetrahydrocannabinol_leads_to_a_rise_in_caspase-3_morphological_changes_in_microglial_and_astrocyte_reactivity_in_the_cerebellum_of_rats\" target=\"_blank\" rel=\"nofollow noopener\"> <button style=\"padding: 10px 20px; font-size: 16px; background-color: #0c3a2e; color: white; border: none; border-radius: 6px; cursor: pointer;\"> Visit Source <\/button> <\/a><\/p><\/blockquote>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Abstract and Figures October 2023 Toxicology Research\u00a012(3) DOI: 10.1093\/toxres\/tfad098 The present study aimed to elucidate the effect of 10 mg\/kg \u03949-tetrahydrocannabinol (THC) on cerebellar neuronal and glial morphology, apoptosis and inflammatory gene expression using a series of histological assays including stereology, Sholl analysis, immunofluorescence and real-time qPCR in male Wistar rats. A decrease in the <\/p>\n","protected":false},"author":1,"featured_media":2815,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[46],"tags":[],"class_list":["post-3589","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-scientific-articles"],"_links":{"self":[{"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/posts\/3589","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/comments?post=3589"}],"version-history":[{"count":7,"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/posts\/3589\/revisions"}],"predecessor-version":[{"id":3607,"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/posts\/3589\/revisions\/3607"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/media\/2815"}],"wp:attachment":[{"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/media?parent=3589"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/categories?post=3589"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/web-frankfurt.de\/Sani\/wp-json\/wp\/v2\/tags?post=3589"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}